Temperature sensitivity caused by missense suppressor supH and amber suppressor supP in Escherichia coli

Mapping of the supP (Su6+) amber suppressor gene in Escherichia coli.

The supP (Su6(+)) amber suppressor gene has been mapped on the clockwise side of the valS locus near min 95 on the Escherichia coli chromosome.

Suppressor-induced structural changes in a missense L-ribulokinase of Escherichia coli.

A suppressor mutation specific for a missense codon in the L-ribulokinase structural gene of the L-arabinose operon of Escherichia coli B/r enhanced L-arabinose utilization by the strain containing the missense codon. Electrophoretic comparisons of the wild-type, missense, and suppressed missense L-ribulokinases indicated that the suppressor changed the structure of the missense kinase, thereby...

Suppressor of phosphofructokinase mutations of Escherichia coli.

The known locus of fructose-6-phosphate kinase mutations (pfkA) in Escherichia coli is at 76 min on the genetic map. We have now found another gene, pfkB, at 33 min, mutation of which suppresses pfkA mutations. The suppression is not informational, and pfkB may be a second gene for fructose-6-phosphate kinase activity.

Case Report of Meningoencephalitis in Calves Caused by Escherichia coli

Inducible amber suppressor for Bacillus subtilis.

An amber suppressor variant of Bacillus subtilis tyrosyl-tRNA was constructed and placed under control of the isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible Pspac promoter. Addition of IPTG resulted in a 50-fold increase in the expression of an rpsD-lacZ fusion containing a UAG amber codon. This system permitted isolation of a conditional lethal mutant which required IPTG for growth.